Objective:-(1)To prepare a temporary mount of onion cells,(2)to record observations and draw labelled diagrams

Materials Required:-
Onion Knife,Forceps,Blade,Slides,Watch glass ,cover slip,brush,
compound microscope,blotting paper,
Safranine ,Glycerine and water.

Procedure:-
(1)Take a piece of onion scale and bend it until it breaks into two.When it breaks,you will notice that a thin membranous structure still connecting the two bits.This membranous structure is called onion peel .

(3)take some water in a watch glass and put the epidermis in it.Make certain that it is not folded or rolled.

(4)Add 1-2 drops of Iodine solution or Safranine solution.

(5)After about 2-3 minutes take the peel and wash in acid water if Iodine solution is used or in plain water If safranine is used.

(6)Take a clean glass slide and put a drop of glycerine almost in the middle of the slide.

(7)Place the stained piece of the peel in the glycerine and put a cover slip on it.

(8)Soak the overflowing fluid from the corners of the coverslip with the help of the blotting paper and clean the side.

(9)Observe the wet mount under the  microscope .

(10)Draw the diagram of the peel and label .

Observations:-

(1)Under Low power of te microscope

1.The rectangular cells of onion  are seen.The regularly arranged components like cells have clear outline.

2.A deeply coloured,round body is seen in side every cell.This is known as Nucleus.

Precautions- Too much of stain should not be used .
      Microscope should be handled carefully.